KIP2 抗体 (CF®488A)
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北京 101111
Quick Overview for KIP2 抗体 (CF®488A) (ABIN7656305)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- p57Kip2 (Mitotic Inhibitor/Suppressor Protein)(57P06), CF488A conjugate
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产品特性
- Recognizes a protein of 57 kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.
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免疫原
- Recombinant full-length human p57Kip2 protein
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亚型
- IgG2b, kappa
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anti-KIP2 (AA 8-37), (N-Term) antibody
适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4073-4074 unconjugated
anti-KIP2 antibody适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.25-0.5 μg/mL for 30 minutes at RT. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: LS174T, Raji, HT29, SK-BR3 cells. Colon or Prostate carcinomas.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- KIP2
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背景
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Synonyms: Beckwith Wiedemann syndrome (WBS), BWCR, Cyclin dependent kinase inhibitor 1C (CDKN1C), KIP2, p57
Gene Symbol: CDKN1C
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分子量
- 57 kDa
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基因ID
- 1028
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UniProt
- P49918
抗原
-
