Recombinant Cytokeratin 19 抗体 (Biotin)
Quick Overview for Recombinant Cytokeratin 19 抗体 (Biotin) (ABIN7656110)
抗原
See all Cytokeratin 19 (KRT19) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) (rKRT19/799), Biotin conjugate
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产品特性
- This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human KRT19 protein
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亚型
- IgG1
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anti-Keratin 19 (KRT19) antibody
KRT19 适用: 人 WB, ELISA, IHC (p), ICC, IP, ICFC 宿主: 小鼠 Monoclonal A53-B-A2 unconjugated
anti-Keratin 19 (KRT19) (AA 245-391) antibodyKRT19 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 19 (KRT19) (AA 245-391) antibodyKRT19 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D1 unconjugated
anti-Keratin 19 (KRT19) (AA 1-403) antibodyKRT19 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 19 (KRT19) (AA 127-322) antibodyKRT19 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 适用: 人, 小鼠, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal KRT19-800 unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 适用: 人 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal KRT19-799 unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 适用: 人 WB, IHC (p), ICC, IP, ICFC 宿主: 小鼠 Monoclonal BA-17 unconjugated
anti-Keratin 19 (KRT19) (AA 241-400) antibodyKRT19 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 19 (KRT19) (AA 274-400) antibodyKRT19 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 8H1A8 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with rat or ferret. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: MCF-7, HeLa, Hep-G2 cells, Breast Cancer
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
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别名
- Keratin 19
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背景
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Synonyms: k19, k1cs, Keratin 19 Keratin Type i 40kD, krt19
Gene Symbol: KRT19
Tissue Expression: Epithelial cells
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分子量
- 40 kDa
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基因ID
- 3880
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UniProt
- P08727
抗原
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