IDH1 抗体 (AA 280-420) (CF®740)
Quick Overview for IDH1 抗体 (AA 280-420) (CF®740) (ABIN7655795)
抗原
See all IDH1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 280-420
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原理
- IDH1(IDH1/1152), CF740 conjugate
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产品特性
- This antibody recognizes a 45 kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant fragment (119 Amino acid residues around aa 280-420) of human IDH1 protein
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亚型
- IgG1, kappa
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anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-414) antibody
IDH1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 74-333) antibodyIDH1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 156-298) antibodyIDH1 适用: 人, 小鼠, 猴 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 7G8A1 unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (N-Term) antibodyIDH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (Center) antibodyIDH1 适用: 人, 小鼠 WB, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 156-298) antibodyIDH1 适用: 人, 小鼠, 猴 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4A4A8 unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 381-413), (C-Term) antibodyIDH1 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 116-143) antibodyIDH1 适用: 人, 小鼠 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB18238 unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 63-90) antibodyIDH1 适用: 人, 小鼠 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 116-143) antibodyIDH1 适用: 人, 小鼠 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 or 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HepG2, HeLa, HT29 or MCF7 cells. Breast, Colon or Prostate Carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
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别名
- IDH1
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背景
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Synonyms: Cytosolic NADP-isocitrate dehydrogenase, Epididymis luminal protein 216, Epididymis secretory protein Li 26, HEL-216, HEL-S-26, ICDH, IDCD, IDH1, IDP, IDPC, Isocitrate dehydrogenase 1 (NADP ) soluble, NADP dependent isocitrate dehydrogenase peroxisomal, NADP( )-specific ICDH, Oxalosuccinate decarboxylase, PICD
Gene Symbol: IDH1
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分子量
- 45-47 kDa
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基因ID
- 3417
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UniProt
- O75874
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途径
- Warburg Effect
抗原
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