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Recombinant Iba1 抗体 (AA 1-146) (CF®488A)

This anti-Iba1 antibody is a 小鼠 单克隆 antibody detecting Iba1 in IHC 和 IHC (fp). Suitable for 人.
产品编号 ABIN7655781
发货至: 中国

Quick Overview for Recombinant Iba1 抗体 (AA 1-146) (CF®488A) (ABIN7655781)

抗原

See all Iba1 (IBA1) 抗体
Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Iba1 antibody is conjugated to CF®488A

应用范围

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Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

RAIF1-1909
  • 抗原表位

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    AA 1-146

    原理

    AIF1 / Iba1 (Microglia Marker) (rAIF1/1909), CF488A conjugate

    产品特性

    Allograft Inflammatory Factor 1 (also known as AIF1 or IBA1) is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Purified recombinant human AIF1 protein (around aa 1-146)

    亚型

    IgG1
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: Lymph Node, Tonsil or Kidney

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % BSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

    别名

    Allograft Inflammatory Factor 1

    背景

    Synonyms: Allograft inflammatory factor 1, AIF1, IBA1

    Gene Symbol: AIF1

    Tissue Expression: Microglia|Monocytes/macrophages

    分子量

    17 kDa

    基因ID

    199

    UniProt

    P55008

    途径

    Smooth Muscle Cell Migration
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