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IFNA2 抗体 (CF®740)

This anti-IFNA2 antibody is a 小鼠 单克隆 antibody detecting IFNA2 in ELISA, WB 和 Func. Suitable for 人.
产品编号 ABIN7655634
发货至: 中国

Quick Overview for IFNA2 抗体 (CF®740) (ABIN7655634)

抗原

See all IFNA2 抗体
IFNA2 (Interferon, alpha 2 (IFNA2))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This IFNA2 antibody is conjugated to CF®740

应用范围

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ELISA, Western Blotting (WB), Functional Studies (Func)

克隆位点

N27
  • 原理

    Interferon alpha-2 (IFNA2) (N27), CF740 conjugate

    产品特性

    This antibody recognizes a protein of 16-27 kDa, identified as human interferon-alphaII (IFN-alphaII). Its epitope maps between aa43-53 of the IFN-alphaII) (total aa172). This MAb is specific for IFN-alpha(II) and does not cross-react with IFN-alpha(I). Epitopes of N27 and N39 MAb s are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-alpha(II) after viral infections. The IFN-alpha family consists of 24 or more genes or pseudo-genes. IFN-alphaII) is one of the two distinct families (I and II) of human IFN-alpha. The alpha interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Purified recombinant human IFN-alpha2

    亚型

    IgG1, kappa
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells in 0.1 mL. Immunofluorescence: 0.5-1 μg/mL. Blocking activity order Ab without azide. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: Stimulated human peripheral blood mononuclear cells

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    IFNA2 (Interferon, alpha 2 (IFNA2))

    别名

    Interferon Alpha-2

    背景

    Synonyms: Alpha 2a interferon, IFN alpha, IFN-alpha-2, IFNA, IFNA2, IFNA2B, Interferon alpha 2a, Interferon alpha 2b, Interferon alpha-2, Interferon alpha-A, LeIF2, LeIFA

    Gene Symbol: IFNA2

    Tissue Expression: Lymphocytes|Monocytes/macrophages

    分子量

    16-27 kDa

    基因ID

    3440

    途径

    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Hepatitis C
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