ICAM-3/CD50 抗体 (CF®740)
Quick Overview for ICAM-3/CD50 抗体 (CF®740) (ABIN7655566)
抗原
See all ICAM-3/CD50 (ICAM3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- CD50 (ICAM-3)(101-1D2), CF740 conjugate
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产品特性
- Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Stimulated human leukocytes
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亚型
- IgG2a, kappa
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anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody
ICAM3 适用: 人 FACS, IHC (p), Func 宿主: 小鼠 Monoclonal MEM-04 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibodyICAM3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-171 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-203) antibodyICAM3 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2D11D1 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 200-547) antibodyICAM3 适用: 人 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-547) antibodyICAM3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F8 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (Biotin)ICAM3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-171 Biotin
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (PE)ICAM3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-171 PE
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 46-197) antibodyICAM3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-130) antibodyICAM3 适用: 人 ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 259-491) antibodyICAM3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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别名
- CD50
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背景
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Synonyms: ICAMR, Intercellular adhesion molecule 3 (ICAM3)
Gene Symbol: ICAM3
Tissue Expression: Lymphocytes|T-cells
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分子量
- 110-160 kDa
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基因ID
- 3385
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UniProt
- P32942
抗原
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