Recombinant ISR-alpha 抗体 (Extracellular Domain) (CF®568)
Quick Overview for Recombinant ISR-alpha 抗体 (Extracellular Domain) (CF®568) (ABIN7655325)
抗原
See all ISR-alpha 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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原理
- Insulin Receptor Alpha (INSR/2277R), CF568 conjugate
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产品特性
- The insulin receptor (INSR) is a heterodimeric protein complex that has an intracellular beta subunit and an extracellular alpha subunit, which is disulfide-linked to a transmembrane segment. The insulin ligand binds to the INSR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to INSR induces phosphorylation of intra-cellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Type 1 diabetes is an autoimmune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant fragment of extracellular domain of human Insulin Receptor alpha
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亚型
- IgG
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anti-Insulin Receptor alpha (ISR-alpha) antibody
ISR-alpha 适用: 人, 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor alpha (ISR-alpha) (AA 701-760) antibodyISR-alpha 适用: 人, 大鼠 IF (cc), IF (p), WB, FACS, IHC (p), ELISA, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor alpha (ISR-alpha) (AA 51-150) antibodyISR-alpha 适用: 人, 大鼠 IF (cc), IF (p), IHC (p), ELISA, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor alpha (ISR-alpha) antibodyISR-alpha 适用: 人 Crys 宿主: 兔 Chimeric Fab 83-7 unconjugated Recombinant Antibody
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Jurkat cells. Human pancreas.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- ISR-alpha (Insulin Receptor alpha (ISR-alpha))
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别名
- Insulin Receptor Alpha
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背景
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Synonyms: CD220, HHF5, HIR A, INSR, Insulin receptor, Insulin receptor subunit alpha, IR
Gene Symbol: INSR
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分子量
- 135 kDa
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基因ID
- 3643
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UniProt
- P06213
抗原
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