HSPG 抗体 (CF®740)
Quick Overview for HSPG 抗体 (CF®740) (ABIN7654934)
抗原
See all HSPG 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Heparan Sulphate Proteoglycan(A7L6), CF740 conjugate
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产品特性
- This MAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan's strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Murine EHS laminin preparation
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亚型
- IgG2a, kappa
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anti-Heparan Sulphate Protoglycans (HSPG) (AA 19-144) antibody
HSPG 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparan Sulphate Protoglycans (HSPG) antibodyHSPG 适用: 人, Cow, 小鼠, 大鼠 IHC, WB, IP, IHC (p), ICC, IHC (fro) 宿主: 大鼠 Monoclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 1-2 μg/mL. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Breast carcinomas, squamous cell carcinomas.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- HSPG (Heparan Sulphate Protoglycans (HSPG))
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别名
- Heparan Sulfate Proteoglycan
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背景
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Synonyms: Heparan sulfate proteoglycan of basement membrane, HSPG, Hspg2, LG3 peptide, Perlecan, PLC, SJS1
Gene Symbol: HSPG2
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分子量
- >400 kDa
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基因ID
- 3339
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UniProt
- P98160
抗原
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