HCG beta 抗体 (CF®740)
Quick Overview for HCG beta 抗体 (CF®740) (ABIN7654771)
抗原
See all HCG beta 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- HCG-beta(hCGb/459), CF740 conjugate
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产品特性
- This MAb reacts with a protein of 22 kDa, identified as β sub-unit of HCG. It does not cross react with the α sub-unit. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). hCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant hCG beta protein
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亚型
- IgG1
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anti-HCG beta (AA 81-165) antibody
适用: 人 ELISA, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-HCG beta antibody适用: 人, 小鼠, 大鼠 ELISA, IHC, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-HCG beta (C-Term) antibody适用: 人 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-HCG beta (AA 101-150) antibody适用: 人 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: JAR or TT Cells. Placenta
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- HCG beta
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背景
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Synonyms: CG-beta, CGB3, CGB5, CGB7, CGB8, Choriogonadotropin Subunit beta, hCGB
Gene Symbol: CGB3
Tissue Expression: Placenta
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分子量
- 22 kDa
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基因ID
- 1082
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UniProt
- P01233
抗原
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