Granulocyte Marker 抗体 (CF®740)
Quick Overview for Granulocyte Marker 抗体 (CF®740) (ABIN7654656)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Granulocyte Marker(BM-1), CF740 conjugate
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产品特性
- Recognizes 183 kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. It reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. This MAb reacts with early precursor and mature forms of human myeloid cells. It is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that it may play a role in the differentiation of myeloid cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human peripheral blood mononuclear cells
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亚型
- IgG1
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anti-Granulocyte Marker antibody
适用: 人 IF, FACS, IHC (f), IHC (p), ISt 宿主: 小鼠 Monoclonal BM-1 unconjugated
anti-Granulocyte Marker antibody适用: 人, Macaque IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal BM-2 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. No special pretreatment is required for staining of formalin/paraffin tissues. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HL60 cells. Bone marrow, lymph node or tonsil
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Granulocyte Marker
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背景
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Synonyms: Myeloid specific protein, p183 DNA-binding cytoplasmic protein
Tissue Expression: Granulocytes|Myeloid cells
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分子量
- 183 kDa
抗原
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