Recombinant Golgi Complex 抗体 (Biotin)
The Biotin-conjugated 兔 单克隆 anti-Golgi Complex antibody (Clone GLG1-2829R) (ABIN7654629) specifically detects Golgi Complex in IHC 和 IHC (fp).
The antibody is reactive with 人 samples.
产品编号 ABIN7654629
发货至:
中国
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北京 101111
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Quick Overview for Recombinant Golgi Complex 抗体 (Biotin) (ABIN7654629)
抗原
Golgi Complex
抗体类型
Recombinant Antibody
适用
人
宿主
兔
克隆类型
单克隆
标记
Biotin
应用范围
Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
克隆位点
GLG1-2829R
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原理
- Golgi Complex (Marker for Human Cells) (GLG1/2829R), Biotin conjugate
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产品特性
- This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- SU-DHL-1 large cell lymphoma cells.
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亚型
- IgG
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with mouse or rat. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Golgi Complex
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背景
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Synonyms: Golgi Complex, Golgi apparatus, Golgi zone
Gene Symbol: Not Known
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分子量
- Not Known
抗原
-
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