Fibronectin 抗体 (CF®740)
Quick Overview for Fibronectin 抗体 (CF®740) (ABIN7654049)
抗原
See all Fibronectin 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Fibronectin(HFN7.1), CF740 conjugate
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产品特性
- Fibronectin is a dimeric glycoprotein of 440 kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose
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亚型
- IgG1, kappa
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anti-Fibronectin (AA 1201-1300) antibody
适用: 人, 大鼠, 小鼠, Cow WB, ELISA, ICC, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 2117-2236) antibody适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 355-558) antibody适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 313-607) antibody适用: 人 WB, IHC, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 1998-2106) antibody适用: 人 WB, IHC, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 2240-2280) antibody适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1B9 unconjugated
anti-Fibronectin (AA 510-689) antibody适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 355-558) antibody适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Fibronectin (AA 2206-2337) antibody适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin antibodyKD Validated 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: SW156 cells or Kidney
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Fibronectin
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背景
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Synonyms: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3
Gene Symbol: FN1
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分子量
- 220 kDa (monomer), 440 kDa (dimer)
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基因ID
- 2335
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UniProt
- P02751
抗原
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