Fibronectin 抗体 (AA 467-595) (CF®568)
Quick Overview for Fibronectin 抗体 (AA 467-595) (CF®568) (ABIN7654046)
抗原
See all Fibronectin 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 467-595
-
原理
- Fibronectin(FN1/2949), CF568 conjugate
-
产品特性
- Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- Recombinant fragment (around aa 467-595) of human fibronectin protein
-
亚型
- IgG1, kappa
-
-
-
-
应用备注
- For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry
-
说明
-
Positive Control: SW156 cells or Kidney
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.1 mg/mL
-
缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
储存条件
- 4 °C
-
储存方法
-
Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
-
有效期
- 24 months
-
-
- Fibronectin
-
背景
-
Synonyms: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3
Gene Symbol: FN1
-
分子量
- 220 kDa (monomer), 440 kDa (dimer)
-
基因ID
- 2335
-
UniProt
- P02751
抗原
-