Fascin 抗体 (CF®740)
Quick Overview for Fascin 抗体 (CF®740) (ABIN7653922)
抗原
See all Fascin (FSCN1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Fascin-1(FSCN1/417), CF740 conjugate
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产品特性
- Recognizes a protein of 55 kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Full length recombinant human FSCN1 protein
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亚型
- IgG2a, kappa
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anti-Fascin (FSCN1) antibody
FSCN1 适用: 人 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal FSCN1-417 unconjugated
anti-Fascin (FSCN1) antibodyFSCN1 适用: 人, 大鼠 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal FSCN1-418 unconjugated
anti-Fascin (FSCN1) (AA 11-110) antibodyFSCN1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fascin (FSCN1) antibodyFSCN1 适用: 人 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal FSCN1-416 unconjugated
anti-Fascin (FSCN1) (AA 394-493) antibodyFSCN1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Fascin (FSCN1) (pSer39) antibodyFSCN1 适用: 人, 小鼠 WB, FACS, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fascin (FSCN1) (AA 322-350) antibodyFSCN1 适用: 人 WB, IHC, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fascin (FSCN1) (AA 2-187) antibodyFSCN1 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fascin (FSCN1) (AA 1-493) antibodyFSCN1 适用: 人 WB, FACS, IF, ELISA, ICC 宿主: 小鼠 Monoclonal AT13D2 unconjugated
anti-Fascin (FSCN1) (AA 93-142) antibodyFSCN1 适用: 人, 小鼠, 大鼠, 豚鼠, 犬, 马, 斑马鱼, 猴, Pig, Hamster WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HeLa, MCF-7, PC-3 or BEWO cells. Hodgkin's lymphoma, Ovarian or Testicular Carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Fascin (FSCN1)
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别名
- Fascin-1
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背景
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Synonyms: 55 kDa actin-bundling protein, FAN1, Fascin homolog 1, Fascin1, FSCN1, HSN, p55, Singed (Drosophila) like (sea urchin fascin homolog like), SNL
Gene Symbol: FSCN1
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分子量
- 55 kDa
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基因ID
- 6624
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UniProt
- Q16658
抗原
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