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Recombinant EpCAM 抗体 (Extracellular Domain) (CF®640R)

This 兔单克隆 antibody specifically detects EpCAM in WB, IHC 和 IHC (fp). It exhibits reactivity toward 人.

产品编号 ABIN7653592

发货至: 中国

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中国
北京 101111

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Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

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Quick Overview for Recombinant EpCAM 抗体 (Extracellular Domain) (CF®640R) (ABIN7653592)

抗原

See all EpCAM (EPCAM) 抗体

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

抗体类型

Recombinant Antibody

适用

人

宿主

兔

克隆类型

单克隆

标记

This EpCAM antibody is conjugated to CF®640R

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

EGP40-2041R

Discover our top product EPCAM Primary Antibody

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AA 100-224, Extracellular Domain

原理

EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF640R conjugate

产品特性

Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

免疫原

Recombinant human EpCAM fragment from extracellular domain (around aa100-224)

亚型

IgG
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 40-260) antibody
EPCAM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 53-150) antibody
EPCAM 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibody
EPCAM 适用: Pig WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibody
EPCAM 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal EGP40-1110 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM 适用: 人 WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal EGP40-1373 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM 适用: 人 FACS, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal EGP40-1384 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibody
EPCAM 适用: 人 FACS, IHC, StM 宿主: 兔 Monoclonal EGP40-1555R unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rEGP40-1372 unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM 适用: 人 WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal EGP40-1372 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibody
EPCAM 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 7B5C10 unconjugated

应用备注

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

说明

Positive Control: HT29 cells or breast tumor

限制

仅限研究用
状态

Liquid

浓度

0.1 mg/mL

缓冲液

PBS, 0.1 % BSA, 0.05 % azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Protect from light

储存条件

4 °C

储存方法

Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light

有效期

24 months
抗原

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

别名

CD326

背景

Synonyms: Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1)
Gene Symbol: TACSTD1
Tissue Expression: Epithelial cells

分子量

40-43 kDa

基因ID

4072

UniProt

P16422