EPX 抗体 (CF®740)
Quick Overview for EPX 抗体 (CF®740) (ABIN7653523)
抗原
See all EPX 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Eosinophil Peroxidase(AHE-1), CF740 conjugate
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产品特性
- Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human eosinophils from a patient with hypereosinophilic syndrome
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亚型
- IgG1, kappa
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anti-Eosinophil Peroxidase (EPX) (AA 140-300) antibody
EPX 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eosinophil Peroxidase (EPX) (AA 1-715) antibodyEPX 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eosinophil Peroxidase (EPX) (AA 400-500) antibodyEPX 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eosinophil Peroxidase (EPX) (Middle Region) antibodyEPX 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Eosinophil Peroxidase (EPX) (Middle Region) antibodyEPX 适用: 人, 小鼠, 大鼠, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: MCF-7 cells. Tonsil
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- EPX (Eosinophil Peroxidase (EPX))
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别名
- Eosinophil Peroxidase
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背景
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Synonyms: Eosinophil peroxidase heavy chain, EPER, EPO, EPP, EPX, EPX PEN, PERE
Gene Symbol: EPX
Tissue Expression: Eosinophils
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分子量
- 70 kDa (55 kDa-Heavy Chain, 15 kDa-Light Chain)
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基因ID
- 8288
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UniProt
- P11678
抗原
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