Recombinant EGFRviii 抗体 (CF®647)
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北京 101111
Quick Overview for Recombinant EGFRviii 抗体 (CF®647) (ABIN7653357)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- EGFRvIII (Epidermal Growth Factor Receptor, Variant III) (GFR/2600R), CF647 conjugate
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产品特性
- EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- KLH conjugate of EGFRvIII-specific, 14-amino acid peptide from the predicted amino acid sequence at the fusion junction (LEEKKGNYVVTDHC)
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亚型
- IgG
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anti-EGFR vIII (EGFRv3) (AA 1-50) antibody
EGFRv3 适用: 人 FACS, WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: A431 cells. Placenta, Breast, Colon or Bladder Carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))
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别名
- EGFRvIII
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背景
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Synonyms: Erbb1 variant III, ERBB1 variant III, HER1 variant III, Proto-oncogene c-ErbB-1 variant III, Receptor Tyrosine Protein Kinase variant III, ErbB1 variant III
Gene Symbol: EGFR
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分子量
- ~145 kDa (vIII variant)
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基因ID
- 1956
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UniProt
- P00533
抗原
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