Desmoglein 1 抗体 (Intracellular) (CF®740)
Quick Overview for Desmoglein 1 抗体 (Intracellular) (CF®740) (ABIN7653108)
抗原
See all Desmoglein 1 (DSG1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Intracellular
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原理
- Desmoglein-1 (DSG1) (27B2), CF740 conjugate
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产品特性
- This antibody recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human desmoglein-1 intracellular domain
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亚型
- IgG1, kappa
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anti-Desmoglein 1 (DSG1) (AA 30-90) antibody
DSG1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) antibodyDSG1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal 32-2B unconjugated
anti-Desmoglein 1 (DSG1) antibodyDSG1 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-544M unconjugated
anti-Desmoglein 1 (DSG1) (AA 384-539) antibodyDSG1 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (AA 1-1049) antibodyDSG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (AA 1-1049) antibodyDSG1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (N-Term) antibodyDSG1 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (AA 800-1049) antibodyDSG1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) (AA 710-961) antibodyDSG1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Desmoglein 1 (DSG1) antibodyDSG1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: A431 or A-375 cells. Skin
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Desmoglein 1 (DSG1)
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别名
- Desmoglein-1
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背景
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Synonyms: Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1
Gene Symbol: DSG1
Tissue Expression: Epidermal cells
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分子量
- 150 kDa (precursor), 160 kDa (mature)
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基因ID
- 1828
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UniProt
- Q02413
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途径
- Cell-Cell Junction Organization
抗原
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