Recombinant Desmocollin 2+3 抗体
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Quick Overview for Recombinant Desmocollin 2+3 抗体 (ABIN7653102)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Desmocollin-2/3(rDSC2/3437)
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产品特性
- Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human DSC2 protein
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亚型
- IgG1, kappa
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anti-Desmocollin 2+3 (Extracellular) antibody
Carrier-free 适用: 人, 小鼠, 大鼠 IF, WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal 7G6 unconjugated
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应用备注
- For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry
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说明
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Positive Control: HeLa cells. Skin tissue.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, no BSA, no azide
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at -20 °C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Desmocollin 2+3
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别名
- Desmocollin-2/3
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背景
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Synonyms: ARVD11, Cadherin family member 2, CDHF2, Desmocollin-2, Desmocollin-3, Desmosomal glycoprotein II and III, Desmosomal glycoprotein II, Desmosomal glycoprotein III, DG2, DGII/III, DSC2, DSC3
Gene Symbol: DSC2
Tissue Expression: Epidermal cells
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分子量
- 110 kDa (DSC2), 100 kDa (DSC3)
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基因ID
- 1824
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UniProt
- Q02487
抗原
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