Chromogranin A 抗体 (CF®740)
Quick Overview for Chromogranin A 抗体 (CF®740) (ABIN7652408)
抗原
See all Chromogranin A (CHGA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Chromogranin A(LK2H10), CF740 conjugate
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产品特性
- Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human pheochromocytoma
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亚型
- IgG1, kappa
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anti-Chromogranin A (CHGA) (AA 15-148) antibody
CHGA 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) (AA 24-154) antibodyCHGA 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CGA-413 unconjugated
anti-Chromogranin A (CHGA) (AA 278-457) antibodyCHGA 适用: 人 IHC, FACS, WB, ELISA 宿主: 小鼠 Monoclonal 5H10B12 unconjugated
anti-Chromogranin A (CHGA) (AA 87-252) antibodyCHGA 适用: 人 FACS, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1A6B6 unconjugated
anti-Chromogranin A (CHGA) (AA 17-446) antibodyCHGA 适用: Pig IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) (AA 278-457) antibodyCHGA 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 1B7A4 unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人, 小鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人, 大鼠, 小鼠, 猴, Pig IHC, StM 宿主: 小鼠 Monoclonal LK2H10 unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人 IHC, StM 宿主: 兔 Monoclonal CHGA-1731R unconjugated Recombinant Antibody
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Does not react with guinea pig, sheep , or rabbit, others not known. Immunohistology formalin-fixed 0.25-0.5 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: PC12 cells. Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Chromogranin A (CHGA)
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别名
- CHGA
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背景
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Synonyms: Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, Vasostatin I or II
Gene Symbol: CHGA
Tissue Expression: Neuroendocrine cells
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分子量
- 68-75 kDa
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基因ID
- 1113
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UniProt
- P10645
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途径
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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