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Recombinant CD45RB 抗体 (CF®740)

This anti-CD45RB antibody is a 兔 单克隆 antibody detecting CD45RB in FACS, IHC, IF, WB 和 IHC (fp). Suitable for 人.
产品编号 ABIN7651601
发货至: 中国

Quick Overview for Recombinant CD45RB 抗体 (CF®740) (ABIN7651601)

抗原

See all CD45RB 抗体
CD45RB

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

  • 101
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  • 1
单克隆

标记

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This CD45RB antibody is conjugated to CF®740

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

PTPRC-2877R
  • 原理

    CD45RB (B-Cell Marker) (PTPRC/2877R), CF740 conjugate

    产品特性

    CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant full-length human PTPRC protein

    亚型

    IgG
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with rat or rhesus monkey. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Western Blot 0.5-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: Raji, Ramos, U-698, or GA-10 cells. Tonsil

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    CD45RB

    别名

    CD45RB

    背景

    Synonyms: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

    Gene Symbol: PTPRC

    Tissue Expression: B-cells

    分子量

    180-220 kDa

    基因ID

    5788

    UniProt

    P08575
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