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CD22 抗体 (AA 52-178) (CF®740)

Name: CD22 抗体 (AA 52-178) (CF®740)
SKU: ABIN7651010
Availability: OutOfStock

This anti-CD22 antibody is a 小鼠单克隆 antibody detecting CD22 in FACS, WB, IHC, IF 和 IHC (fp). Suitable for 人.

产品编号 ABIN7651010

发货至: 中国

Quick Overview for CD22 抗体 (AA 52-178) (CF®740) (ABIN7651010)

抗原

See all CD22 抗体

CD22 (CD22 Molecule (CD22))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This CD22 antibody is conjugated to CF®740

应用范围

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

BLCAM-1795

Discover our top product CD22 Primary Antibody

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AA 52-178

原理

CD22 / BL-CAM (BLCAM/1795), CF740 conjugate

产品特性

This antibody recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

免疫原

Recombinant human BL-CAM/CD22 protein (aa52-178)

亚型

IgG1, kappa
anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 适用: 人 FACS, WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 2H1C4 unconjugated

anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 适用: 人 FACS, WB, ELISA, IHC, IF, Coat, StM 宿主: 小鼠 Monoclonal BLCAM-1795 unconjugated

anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 1A3A11 unconjugated

anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 适用: 人 FACS, WB, ELISA, IHC, IF, Coat 宿主: 小鼠 Monoclonal BLCAM-1796 unconjugated

anti-CD22 Molecule (CD22) antibody
CD22 适用: 人, Non-Human Primate FACS, WB, IP 宿主: 小鼠 Monoclonal MEM-01 unconjugated

anti-CD22 Molecule (CD22) antibody
CD22 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C3 unconjugated

anti-CD22 Molecule (CD22) (AA 20-687) antibody
CD22 适用: 人 FACS, WB, ELISA, IF, IP 宿主: 兔 Monoclonal DM13 unconjugated

anti-CD22 Molecule (CD22) antibody (PE)
CD22 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-01 PE

anti-CD22 Molecule (CD22) antibody (FITC)
CD22 适用: 人 FACS 宿主: 小鼠 Monoclonal IS7 FITC

anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-022R unconjugated Recombinant Antibody

应用备注

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: 2-4 μg/mL for coating order Ab without BSA. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

说明

Positive Control: Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human Tonsil

限制

仅限研究用
状态

Liquid

浓度

0.1 mg/mL

缓冲液

PBS, 0.1 % rBSA, 0.05 % azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Protect from light

储存条件

4 °C

储存方法

Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light

有效期

24 months
抗原

CD22 (CD22 Molecule (CD22))

别名

BL-CAM

背景

Synonyms: B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14
Gene Symbol: CD22
Tissue Expression: B-cells|Lymphocytes

分子量

130-140 kDa

基因ID

933

UniProt

P20273