CD22 抗体 (CF®740)
Quick Overview for CD22 抗体 (CF®740) (ABIN7651008)
抗原
See all CD22 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- CD22(Myg13), CF740 conjugate
-
产品特性
- This antibody ecognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- Human Raji cells
-
亚型
- IgG1, kappa
-
-
anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 适用: 人 FACS, WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 2H1C4 unconjugated
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 适用: 人 FACS, WB, ELISA, IHC, IF, Coat, StM 宿主: 小鼠 Monoclonal BLCAM-1795 unconjugated
anti-CD22 Molecule (CD22) (AA 621-725) antibodyCD22 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 1A3A11 unconjugated
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 适用: 人 FACS, WB, ELISA, IHC, IF, Coat 宿主: 小鼠 Monoclonal BLCAM-1796 unconjugated
anti-CD22 Molecule (CD22) antibodyCD22 适用: 人, Non-Human Primate FACS, WB, IP 宿主: 小鼠 Monoclonal MEM-01 unconjugated
anti-CD22 Molecule (CD22) antibodyCD22 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C3 unconjugated
anti-CD22 Molecule (CD22) (AA 20-687) antibodyCD22 适用: 人 FACS, WB, ELISA, IF, IP 宿主: 兔 Monoclonal DM13 unconjugated
anti-CD22 Molecule (CD22) antibody (PE)CD22 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-01 PE
anti-CD22 Molecule (CD22) antibody (FITC)CD22 适用: 人 FACS 宿主: 小鼠 Monoclonal IS7 FITC
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-022R unconjugated Recombinant Antibody
-
-
应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
-
说明
-
Positive Control: Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human tonsil and lymphocytes.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.1 mg/mL
-
缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
储存条件
- 4 °C
-
储存方法
-
Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
-
有效期
- 24 months
-
-
- CD22 (CD22 Molecule (CD22))
-
别名
- BL-CAM
-
背景
-
Synonyms: B-cell receptor CD22|B-lymphocyte cell adhesion molecule (BL-CAM)|Lectin 2|Lyb8|Sialic acid-binding Ig-like lectin 2 (Siglec-2)|SIGLEC2|T-cell surface antigen Leu-14
Gene Symbol: CD22
Tissue Expression: B-cells|Lymphocytes
-
分子量
- 130-140 kDa
-
基因ID
- 933
-
UniProt
- P20273
抗原
-
