Recombinant CA9 抗体 (CF®405S)
Quick Overview for Recombinant CA9 抗体 (CF®405S) (ABIN7650481)
抗原
See all CA9 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Renal Cell Carcinoma (Carbonic Anhydrase IX) (CA9/2993R), CF405S conjugate
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产品特性
- Carbonic anhydrase IX (carbonic anhydrase 9) is one of several carbolic anhydrases that vary in tissue distribution and localization. Carbonic anhydrases catalyze the interconversion of carbon dioxide and water into carbonic acid and bicarbonate and are found in all mammalian tissues. Carbonic anhydrase IX is a transmembrane protein that is not expressed in most healthy tissues, but is expressed in most renal cell carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human CA9 protein
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亚型
- IgG
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Normal kidney or renal cell carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- CA9 (Carbonic Anhydrase IX (CA9))
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别名
- Carbonic Anhydrase IX
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背景
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Synonyms: Carbonic anhydrase IX, Carbonic anhydrase 9, RCC-associated antigen G250, Carbonic dehydratase, CA-IX, Membrane antigen MN
Gene Symbol: CA9
Tissue Expression: Kidney
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分子量
- 55 kDa
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基因ID
- 768
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UniProt
- Q16790
抗原
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