S100A8/A9 Complex (Calprotectin) 抗体 (CF®740)
Quick Overview for S100A8/A9 Complex (Calprotectin) 抗体 (CF®740) (ABIN7650472)
抗原
See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Calgranulin B / MRP-8/-14 / MAC 387 / S100A8/A9 / Macrophage Ag(MAC387), CF740 conjugate
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产品特性
- Calprotectin is a heteroduplex of Protein S100-A8/A9 (also called MRP-8/14, or Calgranulin A/B). This antibody recognizes the 14 kDa B component (Protein S100-A9) of the complex. Protein S100-A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. Altered expression of this protein is associated with the disease cystic fibrosis. This antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Affinity purified monocyte membrane preparation
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亚型
- IgG1, kappa
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anti-Calprotectin (S100A8/A9) antibody
S100A8/A9 适用: 人, Pig, Cow, Cat, 犬, 豚鼠, 猴, 兔, 大鼠, 小鼠, 马, Baboon, 山羊 IHC, StM 宿主: 小鼠 Monoclonal rMAC3781 unconjugated Recombinant Antibody
anti-Calprotectin (S100A8/A9) antibodyS100A8/A9 适用: 人, Pig, Cow, Cat, 犬, 豚鼠, 猴, 兔, 大鼠, 小鼠, 马, Baboon, 山羊 IHC, StM 宿主: 兔 Monoclonal MAC3157R unconjugated Recombinant Antibody
anti-Calprotectin (S100A8/A9) (AA 1-114) antibodyS100A8/A9 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Calprotectin (S100A8/A9) antibodyAnimal-Free S100A8/A9 适用: 人 ELISA 宿主: 小鼠 Monoclonal AB467-1-A05 unconjugated Recombinant Antibody
anti-Calprotectin (S100A8/A9) antibodyS100A8/A9 适用: 人, Pig, Cow, Cat, 犬, 豚鼠, 猴, 兔, 大鼠, 小鼠, 马, Baboon, 山羊 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal SPM281 unconjugated
anti-Calprotectin (S100A8/A9) antibodyS100A8/A9 适用: 人 IHC, WB, ELISA 宿主: 小鼠 Monoclonal 69-6-1 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Tonsil, lymph node, or spleen
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))
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别名
- Calprotectin
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背景
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Synonyms: Calprotectin, Protein S100-A9, Myeloid-related protein 14, MRP-14, Calgranulin B, CAGB, Calprotectin L1H Subunit
Tissue Expression: Granulocytes|Monocytes/macrophages
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分子量
- 12-14 kDa (doublet)
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基因ID
- 6279
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途径
- S100 Proteins
抗原
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