CNN1 抗体 (CF®740)
Quick Overview for CNN1 抗体 (CF®740) (ABIN7650458)
抗原
See all CNN1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Calponin-1(CNN1/832), CF740 conjugate
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产品特性
- Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle. Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human CNN1 protein
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亚型
- IgG1, kappa
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anti-Calponin 1 (CNN1) (AA 1-268) antibody
CNN1 适用: 人 IHC, WB, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Calponin 1 (CNN1) (AA 201-297) antibodyCNN1 适用: 人, 大鼠, 小鼠 FACS, WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Calponin 1 (CNN1) antibodyCNN1 适用: 人, 大鼠 FACS, IF, IHC, StM 宿主: 兔 Monoclonal CNN1-1408R unconjugated Recombinant Antibody
anti-Calponin 1 (CNN1) antibodyCNN1 适用: 人, 大鼠 FACS, IF, IHC, WB, StM 宿主: 小鼠 Monoclonal CALP unconjugated
anti-Calponin 1 (CNN1) (AA 16-165) antibodyCNN1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1H5B5 unconjugated
anti-Calponin 1 (CNN1) antibodyCNN1 适用: 人, 大鼠 FACS, IHC, WB, StM 宿主: 小鼠 Monoclonal rCNN1-832 unconjugated Recombinant Antibody
anti-Calponin 1 (CNN1) (AA 10-39), (N-Term) antibodyCNN1 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB19472 unconjugated
anti-Calponin 1 (CNN1) (AA 1-297) antibodyCNN1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F5-1H4 unconjugated
anti-Calponin 1 (CNN1) (C-Term) antibodyCNN1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-455R unconjugated Recombinant Antibody
anti-Calponin 1 (CNN1) antibodyCNN1 适用: 人 IHC, WB, ELISA 宿主: 兔 Monoclonal 2H1 unconjugated Recombinant Antibody
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Myoepithelial cells in breast ducts
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- CNN1 (Calponin 1 (CNN1))
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别名
- Calponin-1
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背景
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Synonyms: Calponin-1, CNN1, Calponin 1 basic smooth muscle, Calponin H1 smooth muscle
Gene Symbol: CNN1
Tissue Expression: Smooth muscle
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分子量
- 34 kDa
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基因ID
- 1264
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UniProt
- P51911
抗原
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