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Recombinant Caldesmon 抗体 (High Molecular Weight)

This anti-Caldesmon antibody is a 小鼠 单克隆 antibody detecting Caldesmon in WB, IHC 和 IHC (fp). Suitable for 人.
产品编号 ABIN7650420
发货至: 中国

Quick Overview for Recombinant Caldesmon 抗体 (High Molecular Weight) (ABIN7650420)

抗原

See all Caldesmon (CALD1) 抗体
Caldesmon (CALD1) (Caldesmon 1 (CALD1))

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

  • 100
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单克隆

标记

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This Caldesmon antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

RCALD1-820
  • 抗原表位

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    High Molecular Weight

    原理

    Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (rCALD1/820)

    产品特性

    Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb This antibody recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant full-length human CALD1 protein

    亚型

    IgG1, kappa
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with rat. Immunohistology (formalin): 0.25-0.5 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA pH 7.5-8.5 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, no BSA, no azide

    储存液

    Without preservative

    储存条件

    -20 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Store at -20 °C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    Caldesmon (CALD1) (Caldesmon 1 (CALD1))

    别名

    Caldesmon

    背景

    Synonyms: Caldesmon, Caldesmon 1, Caldesmon 1 Isoform 1, CDM, HCAD

    Gene Symbol: CALD1

    Tissue Expression: Smooth muscle

    分子量

    150 kDa

    基因ID

    800

    UniProt

    Q05682

    途径

    Myometrial Relaxation and Contraction
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