Blood Group Lewis B 抗体 (CF®740)
Quick Overview for Blood Group Lewis B 抗体 (CF®740) (ABIN7650075)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Lewis B(2-25LE), CF740 conjugate
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产品特性
- The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Mucin isolated from a human ovarian cyst fluid
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亚型
- IgG1, kappa
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anti-Blood Group Lewis B antibody
适用: 人 IHC (p), WB, ELISA 宿主: 小鼠 Monoclonal 2-25LE unconjugated
anti-Blood Group Lewis B antibody适用: 人 IHC (p), WB, EIA 宿主: 小鼠 Monoclonal 2-25LE unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Colon
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Blood Group Lewis B
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别名
- Lewis-B
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背景
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Synonyms: Blood group Lewis alpha-4-fucosyltransferase, Fucosyltransferase 3, Fucosyltransferase III, FucT-III, FUT3, Galactoside 3(4)-L-fucosyltransferase, gastric mucin, leB, lewis antigen system, lewis B blood group antigen, Lewis FT, major airway glycoprotein
Gene Symbol: FUT3
Tissue Expression: Epithelial cells
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分子量
- 45 kDa
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基因ID
- 2525
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UniProt
- P21217
抗原
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