Recombinant Biotin 抗体 (CF®405S)
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北京 101111
Quick Overview for Recombinant Biotin 抗体 (CF®405S) (ABIN7650064)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Biotin (Vitamin B7 or Vitamin H) (BTN/2032R), CF405S conjugate
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产品特性
- This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Biotinylated sheep immunoglobulin
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亚型
- IgG
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anti-Biotin antibody
WB, IHC, IP, ICC 宿主: 兔 Monoclonal BLR315M unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, IP, ICC 宿主: 兔 Monoclonal BLR315M unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, ICC 宿主: 兔 Monoclonal BLR328M unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, FACS, ELISA, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibody适用: Various Species IHC 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibody适用: Various Species WB, IHC, FACS, IF 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyWB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. In situ Nucleic Acid Hybridization. No special pretreatment is required for the immunohistochemical staining of formalin-fixed paraffin-embedded tissues.. Western blotting. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Biotinylated proteins in solution or on tissues
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Biotin
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物质类
- Chemical
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背景
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Synonyms: Vitamin B7 or Vitamin H
Gene Symbol: Not Applicable
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分子量
- 244Da
抗原
-
