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Liver Arginase 抗体 (AA 11-97)

This 小鼠 单克隆 anti-Liver Arginase antibody (Clone ARG1-1125) specifically detects Liver Arginase in WB, IHC 和 IHC (fp). The antibody is reactive with 人 samples.
产品编号 ABIN7649682
发货至: 中国
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Room 801-803
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Quick Overview for Liver Arginase 抗体 (AA 11-97) (ABIN7649682)

抗原

See all Liver Arginase (ARG1) 抗体
Liver Arginase (ARG1) (Arginase, Liver (ARG1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Liver Arginase antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

ARG1-1125
  • 抗原表位

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    AA 11-97

    原理

    Arginase 1 (Hepatocellular Carcinoma Marker) (ARG1/1125)

    产品特性

    This antibody recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibodies to Arginase 1 label hepatocytes in normal tissues and granulocytes in peripheral blood. Arginase 1 is a sensitive and specific marker for identification of hepatocellular carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human ARG1 protein fragment (around aa11-97)

    亚型

    IgG3, kappa
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: 293Tcells. Hepatocellular Carcinoma (HCC).

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, no BSA, no azide

    储存液

    Without preservative

    储存条件

    -20 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Store at -20 °C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    Liver Arginase (ARG1) (Arginase, Liver (ARG1))

    别名

    Arginase 1

    背景

    Synonyms: Arginase 1, ARG1, liver-type arginase, type I arginase

    Gene Symbol: ARG1

    Tissue Expression: Liver

    分子量

    35-38 kDa

    基因ID

    383

    UniProt

    P05089

    途径

    Cellular Response to Molecule of Bacterial Origin
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