Liver Arginase 抗体 (AA 11-97) (Biotin)
Quick Overview for Liver Arginase 抗体 (AA 11-97) (Biotin) (ABIN7649676)
抗原
See all Liver Arginase (ARG1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 11-97
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原理
- Arginase 1 (Hepatocellular Carcinoma Marker) (ARG1/1125) , Biotin conjugate
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产品特性
- This antibody recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibodies to Arginase 1 label hepatocytes in normal tissues and granulocytes in peripheral blood. Arginase 1 is a sensitive and specific marker for identification of hepatocellular carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human ARG1 protein fragment (around aa11-97)
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亚型
- IgG3, kappa
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: 293Tcells. Hepatocellular Carcinoma (HCC).
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Liver Arginase (ARG1) (Arginase, Liver (ARG1))
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别名
- Arginase 1
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背景
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Synonyms: Arginase 1, ARG1, liver-type arginase, type I arginase
Gene Symbol: ARG1
Tissue Expression: Liver
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分子量
- 35-38 kDa
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基因ID
- 383
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UniProt
- P05089
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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