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alpha Fetoprotein 抗体 (CF®740)

This anti-alpha Fetoprotein antibody is a 小鼠 单克隆 antibody detecting alpha Fetoprotein in IHC, IF, FACS 和 IHC (fp). Suitable for 人.
产品编号 ABIN7649389
发货至: 中国

Quick Overview for alpha Fetoprotein 抗体 (CF®740) (ABIN7649389)

抗原

See all alpha Fetoprotein (AFP) 抗体
alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

适用

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小鼠

克隆类型

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单克隆

标记

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This alpha Fetoprotein antibody is conjugated to CF®740

应用范围

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Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

C2 C3 MBS-12
  • 原理

    AFP (Alpha Fetoprotein)(C2 + C3 + MBS-12), CF740 conjugate

    产品特性

    Alpha-fetoprotein (AFP) is an oncofetal glycoprotein with a single chain of 70 kDa. This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant full-length human Alpha fetoprotein

    亚型

    IgG
  • 应用备注

    Immunohistology formalin-fixed 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Immunofluorescence 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: HepG2 cells. Fetal liver or hepatocellular carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

    别名

    AFP

    背景

    Synonyms: Alpha-fetoglobulin, FETA, HPAFP

    Gene Symbol: AFP

    分子量

    70 kDa

    基因ID

    174

    UniProt

    P02771

    途径

    C21-Steroid Hormone Metabolic Process
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