Recombinant ACTH 抗体 (AA 1-24) (CF®568)
Quick Overview for Recombinant ACTH 抗体 (AA 1-24) (CF®568) (ABIN7649224)
抗原
See all ACTH 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 1-24
-
原理
- ACTH (Adrenocorticotrophic Hormone) (Pituitary Marker) (CLIP/2040R), CF568 conjugate
-
产品特性
- ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- Synthetic peptide corresponding to aa1-24 of human ACTH
-
亚型
- IgG
-
-
anti-Adrenocorticotropic hormone (ACTH) antibody
ACTH 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3A4H4 unconjugated
anti-Adrenocorticotropic hormone (ACTH) antibodyACTH 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 1A9C6 unconjugated
anti-Adrenocorticotropic hormone (ACTH) (AA 1-39) antibodyACTH 适用: 人, 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Adrenocorticotropic hormone (ACTH) (AA 18-39) antibodyACTH 适用: 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Adrenocorticotropic hormone (ACTH) antibodyACTH 适用: 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
-
说明
-
Positive Control: Normal pituitary gland or pituitary tumor
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.1 mg/mL
-
缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
储存条件
- 4 °C
-
储存方法
-
Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
-
有效期
- 24 months
-
-
- ACTH (Adrenocorticotropic hormone (ACTH))
-
别名
- ACTH
-
背景
-
Synonyms: Pro-opiomelanocortin (POMC), Adrenocorticotropin, Adrenocorticotropic hormone, Melanocyte Stimulating Hormone (MSH), Melanotropin, beta-Endorphin, Lipotropin, Met-Enkephalin, POC
Gene Symbol: POMC
-
分子量
- ACTH is ~5 kDa, and the POMC precursor is ~30 kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.
-
基因ID
- 5443
-
UniProt
- P01189
-
途径
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
抗原
-
