AMPD3 抗体 (CF®740)
Quick Overview for AMPD3 抗体 (CF®740) (ABIN7649180)
抗原
See all AMPD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Adenosine Monophosphate Deaminase 3 (AMPD3)(AMPD3/901), CF740 conjugate
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产品特性
- Adenosine Monophosphate Deaminase, isoform E (AMPD3) is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. It has 767 amino acids, a MW of ~ 90 kDa, and is assigned an EC 3.5.4.6. This MAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This MAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human AMDP3 protein
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亚型
- IgG2b, kappa
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应用备注
- Immunohistology formalin-fixed 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Immunocytochemistry 1-2.0 μg/mL. Immunofluorescence 0.5-1 μg/mL. Flow Cytometry 1-2 μg/million cells in 0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: RBC. Fetal liver, Spleen or Placenta
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- AMPD3 (Adenosine Monophosphate Deaminase 3 (AMPD3))
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别名
- Adenosine Monophosphate Deaminase-3
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背景
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Synonyms: AMPD3, Erythrocyte AMP Deaminase, AMP Deaminase 3
Gene Symbol: AMPD3
Tissue Expression: Red blood cells
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分子量
- 90 kDa
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基因ID
- 272
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UniProt
- Q01432
抗原
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