F-Actin 抗体 (AA 1-50)
This anti-F-Actin antibody is a 兔 多克隆 antibody detecting F-Actin in FACS, WB, ELISA, IHC (p), IF (cc) 和 IF (p). Suitable for 人, 小鼠, 大鼠, 小鸡 和 犬. This Primary Antibody has been cited in 14+ publications.
(14 references)Quick Overview for F-Actin 抗体 (AA 1-50) (ABIN731498)
抗原
F-Actin
适用
人, 小鼠, 大鼠, 小鸡, 犬
宿主
兔
克隆类型
多克隆
标记
This F-Actin antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
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抗原表位
- AA 1-50
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交叉反应
- 小鸡, 犬, 人, 小鼠, 大鼠
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预测反应
- Cow,Sheep,Pig
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit
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亚型
- IgG
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anti-F-Actin (N-Term) antibody
适用: 人, 小鼠, 大鼠, 兔 FACS, WB, IHC (fro), ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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: "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." in: Theriogenology, Vol. 95, pp. 154-162, (2018) (PubMed).
: "The human, F-actin-based cytoskeleton as a mutagen sensor." in: Cancer cell international, Vol. 17, pp. 121, (2018) (PubMed).
: "Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 2030-2036, (2017) (PubMed).
: "Invadopodia proteins, cortactin, N-WASP and WIP differentially promote local invasiveness in ameloblastoma." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 45, Issue 8, pp. 591-8, (2016) (PubMed).
: "Cofilin is correlated with sperm quality and influences sperm fertilizing capacity in humans." in: Andrology, Vol. 4, Issue 6, pp. 1064-1072, (2016) (PubMed).
: "Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice." in: Journal of molecular histology, Vol. 47, Issue 5, pp. 485-90, (2016) (PubMed).
: "Roles of Ras Homolog A in Invasive Ductal Breast Carcinoma." in: Acta histochemica et cytochemica, Vol. 49, Issue 5, pp. 131-140, (2016) (PubMed).
: "Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis." in: Nature cell biology, Vol. 17, Issue 9, pp. 1169-81, (2015) (PubMed).
: "Damage of hippocampal neurons in rats with chronic alcoholism." in: Neural regeneration research, Vol. 9, Issue 17, pp. 1610-5, (2014) (PubMed).
: "Regulation of fibrochondrogenesis of mesenchymal stem cells in an integrated microfluidic platform embedded with biomimetic nanofibrous scaffolds." in: PLoS ONE, Vol. 8, Issue 4, pp. e61283, (2013) (PubMed).
: "A potential gravity-sensing role of vascular smooth muscle cell glycocalyx in altered gravitational stimulation." in: Astrobiology, Vol. 13, Issue 7, pp. 626-36, (2013) (PubMed).
: "In vitro and in vivo evaluations of nano-hydroxyapatite/polyamide 66/glass fibre (n-HA/PA66/GF) as a novel bioactive bone screw." in: PLoS ONE, Vol. 8, Issue 7, pp. e68342, (2013) (PubMed).
: "Hepatoma cell line HepG2.2.15 demonstrates distinct biological features compared with parental HepG2." in: World journal of gastroenterology : WJG, Vol. 17, Issue 9, pp. 1152-9, (2011) (PubMed).
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: "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." in: Theriogenology, Vol. 95, pp. 154-162, (2018) (PubMed).
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- F-Actin
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背景
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Synonyms: CapZ alpha-1, F-actin-capping protein subunit alpha-1, CAZA1_HUMAN
Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions. -
基因ID
- 60
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UniProt
- P52907
抗原
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