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Vinculin 抗体 (Biotin)

This anti-Vinculin antibody is a 兔 多克隆 antibody detecting Vinculin in WB, IHC 和 ICC. Suitable for 人.
产品编号 ABIN7633745
发货至: 中国

Quick Overview for Vinculin 抗体 (Biotin) (ABIN7633745)

抗原

See all Vinculin (VCL) 抗体
Vinculin (VCL)

适用

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宿主

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克隆类型

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多克隆

标记

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This Vinculin antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • 原理

    Biotin-Linked Polyclonal Antibody to Vinculin (VCL)

    特异性

    The antibody is a rabbit polyclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

    纯化方法

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    亚型

    IgG
  • 应用备注

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    说明

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    500 μg/mL

    缓冲液

    PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

    储存液

    Dithiothreitol (DTT), ProClin

    注意事项

    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • 抗原

    Vinculin (VCL)

    别名

    Vinculin

    背景

    MVCL, Metavinculin

    UniProt

    P18206

    途径

    Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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