EIF4E 抗体 (pSer209)
Quick Overview for EIF4E 抗体 (pSer209) (ABIN7603287)
抗原
See all EIF4E 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer209
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原理
- Anti-EIF4E Monoclonal Antibody
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产品特性
- Anti-EIF4E Monoclonal Antibody (ABIN7603287). Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human EIF4E eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
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亚型
- IgG
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应用备注
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WB 1:100-1:5000
IHC 1:100-1:500
IP 1:30
FC 1:40 -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
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浓度
- Lot specific
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
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- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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别名
- EIF4E
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背景
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Synonyms: Transcriptional repressor CTCF,11-zinc finger protein,CCCTC-binding factor,CTCFL paralog,CTCF,
Tissue Specificity: Ubiquitous. Absent in primary spermatocytes. .
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分子量
- 25 kDa
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UniProt
- P06730
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途径
- BCR Signaling
抗原
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