CHD4 抗体 (N-Term)
Quick Overview for CHD4 抗体 (N-Term) (ABIN7603203)
抗原
See all CHD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Anti-CHD4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-CHD4 Antibody Picoband® (ABIN7603203). Tested in WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human CHD4, identical to the related mouse sequences.
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亚型
- IgG
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anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) antibody
CHD4 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1632-1730) antibodyCHD4 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4H4 unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1520-1690) antibodyCHD4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (C-Term) antibodyCHD4 适用: Synthetic WB, ELISA, IF, FACS, Coat, IHC (f) 宿主: 小鼠 Monoclonal 3F2-4 unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1-219) antibodyCHD4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (N-Term) antibodyCHD4 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1-194) antibodyCHD4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3D3E9 unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 71-120) antibodyCHD4 适用: 人, 小鼠, 大鼠, Pig, Cow, 犬, 豚鼠, 马, 兔, Bat, 小鸡, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) antibodyCHD4 适用: 人, 小鼠, 大鼠, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) antibodyCHD4 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal 3F2-4 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Lan, X., Ren, R., Feng, R., Ly, L. C., Lan, Y., Zhang, Z., Aboreden, N., Qin, K., Horton, J. R., Grevet, J. D., Mayuranathan, T., Abdulmalik, O., Keller, C. A., Giardine, B., Hardison, R. C., Crossley, M., Weiss, M. J., Cheng, X., Cheng, X., Shi, J., Blobel, G. A. ZNF410 uniquely activates the NuRD component CHD4 to silence fetal hemoglobin expression. Molec. Cell 81: 239-254, 2021. 2. Le Gallo, M., O'Hara, A. J., Rudd, M. L., Urick, M. E., Hansen, N. F., O'Neil, N. J., Price, J. C., Zhang, S., England, B. M., Godwin, A. K., Sgroi, D. C., NIH Intramural Sequencing Center (NISC) Comparative Sequencing Program, Hieter, P., Mullikan, J. C., Merino, M. J., Bell, D. W. Exome sequencing of serous endometrial tumors identifies recurrent somatic mutations in chromatin-remodeling and ubiquitin ligase complex genes. Nature Genet. 44: 1310-1315, 2012. 3. Ostapcuk, V., Mohn, F., Carl, S. H., Basters, A., Hess, D., Iesmantavicius, V., Lampersberger, L., Flemr, M., Pandey, A., Thoma, N. H., Betschinger, J., Buhler, M. Activity-dependent neuroprotective protein recruits HP1 and CHD4 to control lineage-specifying genes. Nature 557: 739-743, 2018. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))
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别名
- CHD4
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背景
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Synonyms: CHD4, Chromodomain-helicase-DNA-binding protein 4, CHD-4, EC 3.6.4.12, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta
Background: Chromodomain-helicase-DNA-binding protein 4 is an enzyme that in humans is encoded by the CHD4 gene. The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.
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分子量
- 260 kDa
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基因ID
- 1108
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UniProt
- Q14839
抗原
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