MYH10 抗体 (N-Term)
Quick Overview for MYH10 抗体 (N-Term) (ABIN7603186)
抗原
See all MYH10 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
 - N-Term
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原理
 - Anti-non-muscle Myosin IIB/MYH10 Antibody Picoband®
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交叉反应 (详细)
 - No cross-reactivity with other proteins.
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产品特性
 - Anti-non-muscle Myosin IIB/MYH10 Antibody Picoband® (ABIN7603186). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
 - Immunogen affinity purified.
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免疫原
 - A synthetic peptide corresponding to a sequence at the N-terminus of human non-muscle Myosin IIB/MYH10, identical to the related mouse and rat sequences.
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亚型
 - IgG
 
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应用备注
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                        Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse, Rat
1. Ma, X., Adelstein, R. S. A point mutation in Myh10 causes major defects in heart development and body wall closure. Circ. Cardiovasc. Genet. 7: 257-265, 2014. Note: Erratum: Circ. Cardiovasc. Genet. 7: 570 only, 2014. 2. Tuzovic, L., Yu, L., Zeng, W., Li, X., Lu, H., Lu, H.-M., Gonzalez, K., Chung, W. K. A human de novo mutation in MYH10 phenocopies the loss of function mutation in mice. Rare Dis. 1: e26144, 2013. Note: Electronic Article. - 
                                            
限制
 - 仅限研究用
 
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状态
 - Lyophilized
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溶解方式
 - Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
 - 500 μg/mL
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缓冲液
 - Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
 - 4 °C,-20 °C
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储存方法
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                        Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. 
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. 
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- MYH10 (Myosin, Heavy Polypeptide 10, Non-Muscle (MYH10))
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别名
 - MYH10
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背景
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Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, Cd3e
Tissue Specificity: Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.
Background: This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin, it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.
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分子量
 - 229 kDa
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基因ID
 - 4628
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UniProt
 - P35580
 
抗原
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