ALPL 抗体 (N-Term)
Quick Overview for ALPL 抗体 (N-Term) (ABIN7603162)
抗原
See all ALPL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Anti-Alkaline phosphatase/ALPL Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Alkaline phosphatase/ALPL Antibody Picoband® (ABIN7603162). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline phosphatase/ALPL, which shares 76.2% and 81% amino acid (aa) sequence identity with mouse and rat Alkaline phosphatase/ALPL, respectively.
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亚型
- IgG
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anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 18-335) antibody
ALPL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibodyALPL 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2F4 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibodyALPL 适用: 人, Cow IHC, StM 宿主: 小鼠 Monoclonal ALPL-597 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 21-35), (N-Term) antibodyALPL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 217-246) antibodyALPL 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 91-200) antibodyALPL 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 4H1 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (Internal Region) antibodyALPL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 20-270) antibodyALPL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibodyALPL 适用: 人 WB, IHC (p), FACS, EIA 宿主: 小鼠 Monoclonal 2F4 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 52-150) antibodyALPL 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Fedde, K. N., Whyte, M. P. : Alkaline phosphatase (tissue-nonspecific isoenzyme) is a phosphoethanolamine and pyridoxal-5-prime-phosphate ectophosphatase: normal and hypophosphatasia fibroblast study. Am. J. Hum. Genet. 47: 767-775, 1990. 2. Weiss, M. J., Cole, D. E. C., Ray, K., Whyte, M. P., Lafferty, M. A., Mulivor, R. A., Harris, H. : A missense mutation in the human liver/bone/kidney alkaline phosphatase gene causing a lethal form of hypophosphatasia. Proc. Nat. Acad. Sci. 85: 7666-7669, 1988. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
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别名
- ALPL
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背景
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Synonyms: Core-binding factor subunit beta, CBF-beta, Polyomavirus enhancer-binding protein 2 beta subunit, PEA2-beta, PEBP2-beta, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, CBFB
Tissue Specificity: Found only in peripheral nervous system Schwann cells.
Background: Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
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分子量
- 80 kDa
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基因ID
- 249
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UniProt
- P05186
抗原
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