PKM 抗体 (N-Term)
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Quick Overview for PKM 抗体 (N-Term) (ABIN7603151)
抗原
See all PKM 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Anti-PKM2 Antibody (monoclonal, 11I4C3)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PKM2 Antibody (monoclonal, 11I4C3) (ABIN7603151). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.
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亚型
- IgG2b
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anti-Pyruvate Kinase M1/2 (PKM) (AA 11-221) antibody
PKM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 2-531) antibodyPKM 适用: 人 ELISA, WB, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C3C7 unconjugated
anti-Pyruvate Kinase M1/2 (PKM) antibodyPKM 适用: 人 ELISA, WB, IHC, IF, IP, FACS 宿主: 兔 Monoclonal 7B2 unconjugated Recombinant Antibody
anti-Pyruvate Kinase M1/2 (PKM) (AA 11-221) antibodyPKM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 185-461) antibodyPKM 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (Internal Region) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 2-192) antibodyPKM 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibodyPKM 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 1-531) antibodyPKM 适用: 人 ELISA, WB, IF, ICC 宿主: 小鼠 Monoclonal AT1B10 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PKM (Pyruvate Kinase M1/2 (PKM))
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别名
- PKM
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背景
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Synonyms: Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1
Tissue Specificity: Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
Background: PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds ly and selectively to tyrosine-phosphorylated peptides.
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分子量
- 60 kDa
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基因ID
- 5315
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UniProt
- P14618
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途径
- Warburg Effect
抗原
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