Cathepsin F 抗体 (Middle Region)
Quick Overview for Cathepsin F 抗体 (Middle Region) (ABIN7603030)
抗原
See all Cathepsin F (CTSF) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-Cathepsin F/CTSF Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Cathepsin F-CTSF Antibody Picoband® (ABIN7603030). Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Cathepsin F/CTSF, which shares 69.6% amino acid (aa) sequence identity with both mouse Cathepsin F/CTSF.
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亚型
- IgG
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anti-Cathepsin F (CTSF) (AA 249-462) antibody
CTSF 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin F (CTSF) (AA 270-484) antibodyCTSF 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin F (CTSF) (AA 261-290) antibodyCTSF 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19248 unconjugated
anti-Cathepsin F (CTSF) antibodyCTSF 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin F (CTSF) (C-Term) antibodyVerified CTSF 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Cathepsin F (CTSF) (AA 1-484) antibodyCTSF 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cathepsin F (CTSF) antibody (Biotin)CTSF 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal Biotin
anti-Cathepsin F (CTSF) antibody (HRP)CTSF 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal HRP
anti-Cathepsin F (CTSF) antibody (FITC)CTSF 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Di Fabio, R., Moro, F., Pestillo, L., Meschini, M. C., Pezzini, F., Doccini, S., Casali, C., Pierelli, F., Simonati, A., Santorelli, F. M. Pseudo-dominant inheritance of a novel CTSF mutation associated with type B Kufs disease. Neurology 83: 1769-1770, 2014. 2. Nagler, D. K., Sulea, T., Menard, R. Full-length cDNA of human cathepsin F predicts the presence of a cystatin domain at the N-terminus of the cysteine protease zymogen. Biochem. Biophys. Res. Commun. 257: 313-318, 1999. 3. Santamaria, I., Velasco, G., Pendas, A. M., Paz, A., Lopez-Otin, C. Molecular cloning and structural and functional characterization of human cathepsin F, a new cysteine proteinase of the papain family with a long propeptide domain. J. Biol. Chem. 274: 13800-13809, 1999. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Cathepsin F (CTSF)
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别名
- CTSF
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背景
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Synonyms: CTSF, Cathepsin F, CATSF, EC 3.4.22.41
Background: Cathepsin F is a protein that in humans is encoded by the CTSF gene. Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W.
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分子量
- 53 kDa
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基因ID
- 8722
抗原
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