NFIB 抗体 (Middle Region)
Quick Overview for NFIB 抗体 (Middle Region) (ABIN7602995)
抗原
See all NFIB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Middle Region
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原理
- Anti-NFIB/NF1B2 Antibody Picoband® (monoclonal, 4D6E4)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NFIB/NF1B2 Antibody Picoband® (monoclonal, 4D6E4) (ABIN7602995). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human NFIB/NF1B2, identical to the related mouse and rat sequences.
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亚型
- IgG2b
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anti-Nuclear Factor I/B (NFIB) (AA 1-420) antibody
NFIB 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 2D6 unconjugated
anti-Nuclear Factor I/B (NFIB) antibodyNFIB 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/B (NFIB) antibodyNFIB 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/B (NFIB) (AA 1-420) antibodyNFIB 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Nuclear Factor I/B (NFIB) (C-Term) antibodyNFIB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/B (NFIB) antibodyNFIB 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/B (NFIB) (AA 1-233) antibodyNFIB 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/B (NFIB) (AA 187-236) antibodyNFIB 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔, 绵羊, Bat, 小鸡, Hamster, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/B (NFIB) antibodyNFIB 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Nuclear Factor I/B (NFIB) (AA 255-283) antibodyNFIB 适用: 人 WB 宿主: 兔 Polyclonal RB36790 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse, Rat
1. Gründer, Albert, Ebel, Thorsten T., Mallo, Moisés, Schwarzkopf, Georg, Shimizu, Takehiko, Sippel, Albrecht E., Schrewe, Heinrich (2002-03-01). "Nuclear factor I-B (Nfib) deficient mice have severe lung hypoplasia". Mechanisms of Development. 112 (1-2): 69-77. 2. Steele-Perkins, George, Plachez, Céline, Butz, Kenneth G., Yang, Guanhu, Bachurski, Cindy J., Kinsman, Stephen L., Litwack, E. David, Richards, Linda J., Gronostajski, Richard M. (2005-01-15). "The Transcription Factor Gene Nfib Is Essential for both Lung Maturation and Brain Development". Molecular and Cellular Biology. 25 (2): 685-698. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- NFIB (Nuclear Factor I/B (NFIB))
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别名
- NFIB
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背景
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Synonyms: Adhesion molecule antibody, CD31 antibody, CD31 antigen antibody, CD31 EndoCAM antibody, Endocam antibody, FLJ34100 antibody, FLJ58394 antibody, GPIIA antibody, GPIIA' antibody, PECA1 antibody, PECA1_HUMAN antibody, Pecam 1 antibody, PECAM 1 CD31 EndoCAM antibody, PECAM antibody, PECAM-1 antibody, Pecam1 antibody, Platelet endothelial cell adhesion molecule antibody, Platelet/endothelial cell adhesion molecule 1 antibody, Platelet/endothelial cell adhesion molecule antibody
Tissue Specificity: Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level). Expressed on neutrophils (at protein level). Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level).
Background: Nuclear factor 1 B-type is a protein that in humans is encoded by the NFIB gene. The NFIB gene is a part of the NFI gene complex that includes three other genes (NFIA, NFIC and NFIX). The NFIB gene is a protein coding gene that also serves as a transcription factor. This gene is essential in embryonic development and it works together with its gene complex to initiate tissue differentiation in the fetus. Through knockout experiments, researchers found that mice without the NFIB gene have severely underdeveloped lungs. This mutation does not seem to cause spontaneous abortions because in utero the fetus does not use its lungs for respiration. However, this becomes lethal once the fetus is born and has to take its first breath. It is thought that NFIB plays a role in down regulating the transcription factors TGF-β1 and Shh in normal gestation because they remained high in knockout experiments. The absence of NFIB also leads to insufficient amounts of surfactant being produced which is one reason why the mice cannot breathe once it is born. The knockout experiments demonstrated that NFIB has a significant role in fore-brain development. NFIB is typically found in pontine nuclei of the CNS, the cerebral cortex and the white matter of the brain and without NFIB these areas are dramatically affected.
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分子量
- 68 kDa
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基因ID
- 4781
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UniProt
- O00712
抗原
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