ENO1 抗体 (Middle Region)
Quick Overview for ENO1 抗体 (Middle Region) (ABIN7602977)
抗原
See all ENO1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-ENO1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ENO1 Antibody Picoband® (ABIN7602977). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human ENO1, which shares 90.9% amino acid (aa) sequence identity with rat ENO1.
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亚型
- IgG
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anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 适用: 人 WB, ELISA, IF, IP, IHC (p) 宿主: 小鼠 Monoclonal 8G8 unconjugated
anti-Enolase 1 (ENO1) (AA 2-434) antibodyENO1 适用: 人 WB, IHC, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 8H9G12 unconjugated
anti-Enolase 1 (ENO1) (AA 1-434) antibodyENO1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Enolase 1 (ENO1) antibodyENO1 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Enolase 1 (ENO1) (AA 1-434) antibodyENO1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Enolase 1 (ENO1) (Middle Region) antibodyENO1 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 豚鼠, 兔, 马, 犬, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enolase 1 (ENO1) (AA 405-434), (C-Term) antibodyENO1 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB19995 unconjugated
anti-Enolase 1 (ENO1) (AA 246-295) antibodyENO1 适用: 人, 马, 猴, Pig, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Enolase 1 (ENO1) antibodyKO Validated ENO1 适用: 人 WB, IF, ICC, IHC (p), DB, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Enolase 1 (ENO1) (AA 1-434) antibodyENO1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 8G3 unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Comings, D. E. Evidence for ancient tetraploidy and conservation of linkage groups in mammalian chromosomes. Nature 238: 455-457, 1972. 2. D'Ancona, G. G., Chern, C. J., Benn, P., Croce, C. M. Assignment of the human gene for enolase 1 to region pter-p36 of chromosome 1. Cytogenet. Cell Genet. 18: 327-332, 1977. 3. D'Ancona, G. G., Croce, C. M. Assignment of the gene for enolase to mouse chromosome 4 using somatic cell hybrids. Cytogenet. Cell Genet. 19: 1-6, 1977. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ENO1 (Enolase 1 (ENO1))
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别名
- ENO1
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背景
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Synonyms: Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1
Tissue Specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Background: Enolase 1 (ENO1) is a glycolytic enzyme expressed in most tissues. It is mapped to 1p36.23. This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
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分子量
- 47 kDa
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基因ID
- 2023
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UniProt
- P06733
抗原
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