Anti-HSPA4 Antibody Picoband® (ABIN7602896). Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
纯化方法
Immunogen affinity purified.
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human HSPA4, which shares 95.2% amino acid (aa) sequence identity with both mouse and rat HSPA4.
Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human 1. Fathallah, D. M., Cherif, D., Dellagi, K., Arnaout, M. A. Molecular cloning of a novel human hsp70 from a B cell line and its assignment to chromosome 5. J. Immun. 151: 810-813, 1993. Note: Erratum: J. Immun. 151: 6616 only, 1993. 2. Gross, M. B. Personal Communication. Baltimore, Md. 10/22/2020. 3. Mohamed, B. A., Barakat, A. Z., Held, T., Elkenani, M., Muhlfeld, C., Manner, J., Adham, I. M. Respiratory distress and early neonatal lethality in Hspa4l/Hspa4 double-mutant mice. Am. J. Resp. Cell Molec. Biol. 50: 817-824, 2014.
限制
仅限研究用
状态
Lyophilized
溶解方式
Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
抗原
HSPA4
(Heat Shock 70kDa Protein 4 (HSPA4))
别名
HSPA4
背景
Heat shock 70 kDa protein 4 is a protein that in humans is encoded by the HSPA4 gene. The heat shock protein HSPA4 (Apg-2, HSP70RY) is a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 heat-shock proteins. Apg-2 has chaperone ability similiar to Hsp110, and it plays a role under non-stress conditions. Apg-2 interacts with TJP1/ZO-1 and functions as a regulator of ZO-1-ZONAB signaling in epithelial cells in response to cellular stress.