METTL3 抗体 (C-Term)
Quick Overview for METTL3 抗体 (C-Term) (ABIN7602808)
抗原
See all METTL3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-METTL3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-METTL3 Antibody Picoband® (ABIN7602808). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human METTL3, which shares 100% and 95.3% amino acid (aa) sequence identity with mouse and rat METTL3, respectively.
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亚型
- IgG
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anti-Methyltransferase Like 3 (METTL3) antibody
METTL3 适用: 人, 大鼠, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (AA 1-156) antibodyMETTL3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (AA 1-580) antibodyMETTL3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (N-Term) antibodyMETTL3 适用: 人, 大鼠, 小鼠, 犬, 兔, 马, 豚鼠, Cow, 斑马鱼, Bat, Hamster, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (C-Term) antibodyMETTL3 适用: 人, 大鼠, 小鼠, 犬, 兔, 马, 豚鼠, Cow, 斑马鱼, 山羊, Pig, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) antibodyMETTL3 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) antibodyMETTL3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (Internal Region) antibodyMETTL3 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (AA 206-255) antibodyMETTL3 适用: 人, 大鼠, 小鼠, 犬, 兔, 马, 豚鼠, Cow, 斑马鱼, 山羊, Pig, Bat, Hamster, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyltransferase Like 3 (METTL3) (AA 503-552) antibodyMETTL3 适用: 人, 大鼠, 小鼠, 犬, 兔, 马, 豚鼠, Cow, 斑马鱼, Pig, Bat, Hamster, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Alarcon, C. R., Lee, H., Goodarzi, H., Halberg, N., Tavazoie, S. F. N(6)-methyladenosine marks primary microRNAs for processing. Nature 519: 482-485, 2015. 2. Barbieri, I., Tzelepis, K., Pandolfini, L., Shi, J., Millan-Zambrano, G., Robson, S. C., Aspris, D., Migliori, V., Bannister, A. J., Han, N., De Braekeleer, E., Ponstingl, H., Hendrick, A., Vakoc, C. R., Vassiliou, G. S., Kouzarides, T.Promoter-bound METTL3 maintains myeloid leukaemia by m(6)A-dependent translation control. Nature 552: 126-131, 2017. 3. Bujnicki, J. M., Feder, M., Radlinska, M., Blumenthal, R. M. Structure prediction and phylogenetic analysis of a functionally diverse family of proteins homologous to the MT-A70 subunit of the human mRNA:m6A methyltransferase. J. Molec. Evol. 55: 431-444, 2002. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- METTL3 (Methyltransferase Like 3 (METTL3))
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别名
- METTL3
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背景
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Synonyms: N6-adenosine-methyltransferase catalytic subunit
Tissue Specificity: Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Background: N6-adenosine-methyltransferase 70 kDa subunit (METTL3) is an enzyme that in humans is encoded by the METTL3 gene. It is mapped to 14q11.2. This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
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分子量
- 75 kDa
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基因ID
- 56339
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UniProt
- Q86U44
抗原
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