PDIA3 抗体 (C-Term)
Quick Overview for PDIA3 抗体 (C-Term) (ABIN7602778)
抗原
See all PDIA3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- Anti-ERp57 Antibody Picoband® (monoclonal, 7E5)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ERp57 Antibody Picoband® (monoclonal, 7E5) (ABIN7602778). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human ERp57, different from the related mouse and rat sequences by two amino acids.
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亚型
- IgG1
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anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (C-Term) antibody
PDIA3 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 猴, Cow, 兔, Pig, Hamster, 马, 山羊, 绵羊, Avian, Cat, 小鸡, 驴 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 1-505) antibodyPDIA3 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 140-505) antibodyPDIA3 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3H5 unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) antibodyPDIA3 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3E1 unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (C-Term) antibodyPDIA3 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) antibodyPDIA3 适用: 人 WB, IHC, IF, IP, ICC 宿主: 小鼠 Monoclonal Map-ERp57 unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 1-505) antibodyPDIA3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) antibody (HRP)PDIA3 适用: 人 WB, IHC, IF, IP, ICC 宿主: 小鼠 Monoclonal Map-ERp57 HRP
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) antibody (FITC)PDIA3 适用: 人 WB, IHC, IF, IP, ICC 宿主: 小鼠 Monoclonal Map-ERp57 FITC
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) antibody (Biotin)PDIA3 适用: 人 WB, IHC, IF, IP, ICC 宿主: 小鼠 Monoclonal Map-ERp57 Biotin
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene: PDIA3 protein disulfide isomerase family A, member 3". 2. Ellerman, D. A., Myles, D. G., Primakoff, P. A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57. Dev. Cell 10: 831-837, 2006 3. Garbi, N., Tanaka, S., Momburg, F., Hammerling, G. J. Impaired assembly of the major histocompatibility complex class I peptide-loading complex in mice deficient in the oxidoreductase ERp57. Nature Immun. 7: 93-102, 2006. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
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别名
- PDIA3
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背景
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Synonyms: Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, K8, Type-II keratin Kb8, KRT8, CYK8
Tissue Specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Background: PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme. It is mapped on 15q15.3. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase, however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
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分子量
- 57 kDa
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基因ID
- 2923
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UniProt
- P30101
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途径
- Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis
抗原
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