Acetyl-CoA Carboxylase beta 抗体 (C-Term)
Quick Overview for Acetyl-CoA Carboxylase beta 抗体 (C-Term) (ABIN7602757)
抗原
See all Acetyl-CoA Carboxylase beta (ACACB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband® (ABIN7602757). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human Acetyl Coenzyme A Carboxylase/ACACB, which shares 64.7% amino acid (aa) sequence identity with mouse Acetyl Coenzyme A Carboxylase/ACACB.
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亚型
- IgG
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anti-Acetyl-CoA Carboxylase beta (ACACB) (AA 29-249) antibody
ACACB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Acetyl-CoA Carboxylase beta (ACACB) (Internal Region) antibodyRecent ACACB 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Acetyl-CoA Carboxylase beta (ACACB) (Internal Region) antibodyVerified ACACB 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Acetyl-CoA Carboxylase beta (ACACB) (AA 22-120) antibodyACACB 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1C11 unconjugated
anti-Acetyl-CoA Carboxylase beta (ACACB) (N-Term) antibodyACACB 适用: 人, 小鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Widmer J, Fassihi KS, Schlichter SC, Wheeler KS, Crute BE, King N, Nutile-McMenemy N, Noll WW, Daniel S, Ha J, Kim KH, Witters LA (June 1996). "Identification of a second human acetyl-CoA carboxylase gene". The Biochemical Journal. 316 ( Pt 3) (3): 915-22. 2. Corbett JW, Harwood JH (November 2007). "Inhibitors of mammalian acetyl-CoA carboxylase". Recent Patents on Cardiovascular Drug Discovery. 2 (3): 162-80. 3. Olson DP, Pulinilkunnil T, Cline GW, Shulman GI, Lowell BB (April 2010). "Gene knockout of Acc2 has little effect on body weight, fat mass, or food intake". Proceedings of the National Academy of Sciences of the United States of America. 107 (16): 7598-603. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Acetyl-CoA Carboxylase beta (ACACB)
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别名
- ACACB
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背景
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Synonyms: Acetyl-CoA carboxylase 2, ACC-beta, Biotin carboxylase, ACACB, ACC2, ACCB
Tissue Specificity: Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon (PubMed:9099716). Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level) (PubMed:19190759). Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis (PubMed:19190759).
Background: Acetyl-CoA carboxylase 2 also known as ACC-beta or ACC2 is an enzyme that in humans is encoded by the ACACB gene. It is mapped to 12q24.11. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms.
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分子量
- 277 kDa
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基因ID
- 32
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UniProt
- O00763
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途径
- AMPK Signaling, Ribonucleoside Biosynthetic Process
抗原
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