WAPAL 抗体 (AA 916-1190)
Quick Overview for WAPAL 抗体 (AA 916-1190) (ABIN7602663)
抗原
See all WAPAL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 916-1190
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原理
- Anti-WAPL/FOE Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-WAPL/FOE Antibody Picoband® (ABIN7602663). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human WAPL/FOE recombinant protein (Position: Q916-C1190).
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亚型
- IgG
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anti-Wings Apart-Like Protein Homolog (WAPAL) (AA 1-402) antibody
WAPAL 适用: 人 ELISA, WB, IP 宿主: 小鼠 Monoclonal 2B3 unconjugated
anti-Wings Apart-Like Protein Homolog (WAPAL) antibodyWAPAL 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Wings Apart-Like Protein Homolog (WAPAL) (AA 1037-1190) antibodyWAPAL 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Wings Apart-Like Protein Homolog (WAPAL) (AA 1-402) antibodyWAPAL 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Wings Apart-Like Protein Homolog (WAPAL) (AA 731-830) antibodyWAPAL 适用: 人 ELISA, WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Wings Apart-Like Protein Homolog (WAPAL) (AA 1-402) antibodyWAPAL 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Busslinger, G. A., Stocsits, R. R., van der Lelij, P., Axelsson, E., Tedeschi, A., Galjart, N., Peters, J.-M. Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl. Nature 544: 503-507, 2017. 2. Hill, L., Ebert, A., Jaritz, M., Wutz, G., Nagasaka, K., Tagoh, H., Kostanova-Poliakova, D., Schindler, K., Sun, Q., Bonelt, P., Fischer, M., Peters, J.-M., Busslinger, M. Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion. Nature 584: 142-147, 2020. 3. Kueng, S., Hegemann, B., Peters, B. H., Lipp, J. J., Schleiffer, A., Mechtler, K., Peters, J.-M. Wapl controls the dynamic association of cohesin with chromatin. Cell 127: 955-967, 2006. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- WAPAL (Wings Apart-Like Protein Homolog (WAPAL))
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别名
- WAPL
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背景
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Synonyms: p-selectin glycoprotein ligand, Selplg, Psgl1
Tissue Specificity: Highly expressed in blood, bone marrow, brain, adipose tissue, spleen, and thymus. Also expressed in heart, kidney, liver, muscle, ovary, and stomach.
Background: WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth. It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by ly interacting with its regulatory subunits. WAPL is a new regulator of the development and metastasis of cancerous tissue.
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分子量
- 180 kDa
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基因ID
- 23063
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UniProt
- Q7Z5K2
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途径
- Chromatin Binding
抗原
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