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EIF4G2 抗体 (AA 91-790)

This anti-EIF4G2 antibody is a 兔 多克隆 antibody detecting EIF4G2 in WB, ELISA, IF, ICC 和 FACS. Suitable for 人, 小鼠, 大鼠 和 猴.
产品编号 ABIN7602661
发货至: 中国

Quick Overview for EIF4G2 抗体 (AA 91-790) (ABIN7602661)

抗原

See all EIF4G2 抗体
EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

适用

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人, 小鼠, 大鼠, 猴

宿主

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克隆类型

  • 63
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多克隆

标记

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This EIF4G2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • 抗原表位

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    AA 91-790

    原理

    Anti-P97/DAP5/EIF4G2 Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-P97/DAP5/EIF4G2 Antibody Picoband® (ABIN7602661). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human P97/DAP5/EIF4G2 recombinant protein (Position: E91-E790).

    亚型

    IgG
  • 应用备注

    Western blot, 0.25-0.5 μg/mL, Human, Monkey, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Imataka, H., Olsen, H. S., Sonenberg. N. A new translational regulator with homology to eukaryotic translation initiation factor 4G. EMBO J. 16: 817-825, 1997. 2. Levy-Strumpf, N., Deiss, L. P., Berissi, H., Kimchi, A. DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death. Molec. Cell. Biol. 17: 1615-1625, 1997. 3. Marash, L., Liberman, N., Henis-Korenblit, S., Sivan, G., Reem, E., Elroy-Stein, O., Kimchi, A. DAP5 promotes cap-independent translation of Bcl-2 and CDK1 to facilitate cell survival during mitosis. Molec. Cell 30: 447-459, 2008.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

    别名

    EIF4G2

    背景

    Synonyms: Kallikrein-2, Glandular kallikrein-1, hGK-1, Tissue kallikrein-2, KLK2,

    Background: Eukaryotic translation initiation factor 4 gamma 2 (also called p97, NAT1, and DAP-5) is a protein that in humans is encoded by the EIF4G2 gene. Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3, eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

    分子量

    97 kDa

    基因ID

    1982

    UniProt

    P78344
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